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Convert your PDF files to TIFF image. PDF (Portable Document Format) is a formatting language developed by Adobe, an extensible page-description protocol that implements the native file format based on PostScript language, uses standard compression algorithm, documents can contain text, graphics, multimedia, custom data types and more. Convert your PDF files to TIFF image. PDF (Portable Document Format) is a formatting language developed by Adobe, an extensible page-description protocol that implements the native file format based on PostScript language, uses standard compression algorithm, documents can contain text, graphics, multimedia, custom data types and more. Unfortunately, Adobe Reader cannot convert PDF to TIFF. As a result, you will need to look for an alternative PDF to TIFF Adobe Reader convert. In this case, use PDFelement. This software is compatible with Windows and Mac operating systems. With this software, you can convert your PDF file to image formats like TIFF, BMP, PNG, JPEG, and GIF.

The File / Import submenu

Most of the commands listed, particularly Fiji extensions, are also available (via ImageJ's HandleExtraFileTypes feature) simply by using the File / Open... menu item.

Built-in formats

Fiji primarily uses TIFF as the image file format. The menu command File › Save will save in TIFF format. The menu command File › Open will open TIFF files and import a number of other common file formats (e.g. JPEG, GIF, BMP, PGM, PNG) as well as those detailed below. Files can be also dragged and dropped to the toolbar to open them.

Bio-Formats

Many more file formats can be imported via Fiji plugins. One plugin is called Bio-Formats. This will import and export a wide number of file formats along with their important metadata. They can be accessed directly via the menu command File › Import › Bio-Formats or indirectly via the File › Open menu command which will use the Bio-Formats plugin if needed. See the list of supported file formats on the Bio-Formats website for the complete list (135 as of this writing).

If Bio-Formats is used, you will be prompted with a big dialog and your preferences remembered.

Drag and dropping an image file to the Fiji toolbar will effectively open the file the same as the File › Open command.

Some image file formats are more akin to databases rather than images (e.g. Leica LIF files). In these cases a second dialog will list the images in the 'database' prompting you to select which to open.

There are several different Bio-Formats commands for importing data into Fiji:

• Bio-Formats Remote Importer. For this selection, the image doesn’t have to be saved onto your computer or a disk. But it is a limited feature; it is more robust to download the file and then use the regular Bio-Formats instead.
• Bio-Formats Windowless Importer. With this option, Fiji uses the settings that were previously used for Bio-Formats, so it skips all dialog boxes at the beginning.
• Bio-Formats Importer. Select this command if you need to change the settings for importing an image.

Zeiss LSM panel

The LSM panel plugin duplicates the Zeiss LSM file import function of the LOCI Bio-Format Importer, but will also extensively catalogue the LSM metadata. The LSM Toolbox is activated by the menu command File › Import › LSM. Once you select LSM the panel will appear.

Images are opened as 8-bit color images with the “no-palette” pseudo color from the LSM acquisition software. Each channel is imported as a separate image/stack. Lambda stacks are therefore imported as multiple images, not a single stack. They can be converted to a stack with the menu command: Image › Stacks › Images to stack.

Once opened, the file information can be accessed and the information can be irreversibly stamped into the images or exported.

Multiple files from a folder

Each time point of an experiment acquired with software such as Perkin Elmer’s UltraVIEW or Scion Image’s time lapse macro is saved by the acquisition software as a single TIF. The experimental sequence can be imported to Fiji via the menu command File › Import › Image Sequence.

Convert .1sc File To Tiff Size

Locate the directory, click on the first image in the sequence and OK all dialogs. (You may get a couple of error messages while Fiji tries to open any non-image files in the experimental directory.) The stack will “interleave” the multiple channels you recorded, and can be de-interleaved via Plugins › Stacks - Shuffling › Deinterleave

Selected images that are not the same size can be imported as individual images windows using File/Import and select each individual image you want imported into Fiji. Then you can put those images into a stack by Image › Stacks › Images to stack Unlike the File › Import › Image Sequence function, the images don’t need to be the same dimensions. If memory is limited, stacks can be opened as Virtual-Stacks with most of the stack remaining on the disk until it is required.

Multi-RAW sequence from a folder

To form an image, Fiji needs to know the image dimensions, bit-depth, slice number per file and any extraneous information in the file format (offset and header size). All you really need to tell it is the image dimension in x and y. These values should be obtainable from the software in which the images were acquired. Armed with this information follow these steps:

1. File/Import/Raw…
2. Select experimental directory.
3. Typical values for the dialog box are:
   • Image type = 16-bit unsigned (or 8 bit typically)
   • Width and height as determined earlier
   • Offset = 0, number of image = 1, gap = 0, ‘white’ is zero = off
   • ‘Little-endian byte order’ = on, ‘open all files in folder’ = on to open all files in folder.

Non-images will also be opened and may appear as blank images and need deleting: Image › Stacks › Delete slice. The stack will 'interleave” the multiple channels you recorded, and can be de-interleaved via Plugins › Stacks - Shuffling › DeInterleave.

AVI and MOV files

There are two plugins which can open uncompressed AVIs and some types of MOV file. AVIs can be opened via File › Import › AVI

Convert .1sc File To Tiff Form

Other Import functions

Animated GIF- This plugin opens animated GIF files as an RGB stack. Also opens single GIF images.

Fiji Extensions

• Amira...: Open as a stack both Amira (.am) label and image stack files.
• Biorad...: Open a Biorad (.pic) confocal stack.
• Psychomorph .tem Reader
• Show Amira Surface: Open in a 3D Window Amira .surf surface files (3D meshes).
• DF3...: Open a POV-Ray density file.
• MRC Leginon ...: Open an .mrc image (8, 16 and 32-bit) generated by Leginon (software for automated imaging in transmission electron microscopy).
• PDF ...: Open a .pdf file. Optionally specify which page (or all) and at which scale (from 100% to 1000%). Note that when opening from 'File / Open', 'File / Import / URL' or from a ij.io.Opener().open() method call, this plugin will by default open all pages at 100% magnification.
• Extract Images From PDF...: Extract all images from a PDF file, in their original resolution.
• DAT EMMENU ...: Open a .dat image file from EMMENU software for imaging with transmission electron microscopes.
• DM3 Reader...: Open a .dm3 image file from Gatan cameras, with all its metadata (transmission electron microscopy).
• TorstenRaw GZ Reader...: Open the files generated by the registration tools from http://flybrain.stanford.edu/
• IPLab Reader...
• Animated Gif...: Open an animated .gif file as a stack.
• Amira as TrakEM2...: Open an amira .am image stack file as a TrakEM2 project. The labels file will be asked for. All calibrations and parameters are set to streamline the process.
• Analyze...
• Show LSM Toolbox: Open the LSM Toolbox window, from which LSM files can be opened, viewed in multi dimensions, montaged, their metadata directly inspected, time and z stamped and more.

1. Select a JPG file (such as *.jpg, *.jpeg, *.jp2).
2. Click button 'Convert' to start upload your file.
3. Once upload completed, converter will redirect a web page to show the conversion result.

• If file upload process takes a very long time or no response or very slow, please try to cancel then submit again.
• This converter cannot support encrypted or protected image files.
• If you selected options, be sure to enter valid values.
• If file source is a URL, be sure to enter a complete and valid URL to your file.

• JPG (JPEG Image) is a lossy image compression format, compression method is usually lossy, based on the discrete cosine transform (DCT), encodings include: Sequential Encoding, Progressive Encoding, Lossless Encoding and Hierarchical Encoding. The file extensions can be .jpg, .jpeg, .jp2.
• TIFF (Tagged Image File Format) is a raster graphics image format, allows for a flexible set of information fields, and allows for a wide range of different compression schemes and color spaces, widely used in desktop publishing. The file extensions can be .tiff, .tif.

• JPG to PDF
  PDF (Portable Document Format)
• JPG to BMP
  BMP (Bitmap)
• JPG to PNG
  PNG (Portable Network Graphics)
• JPG to GIF
  GIF (Graphics Interchange Format)
• JPG to WEBP
  WEBP (Web Picture)

• PDF to TIFF
  PDF (Portable Document Format)
• BMP to TIFF
  BMP (Bitmap)
• PNG to TIFF
  PNG (Portable Network Graphics)
• GIF to TIFF
  GIF (Graphics Interchange Format)
• CR2 to TIFF
  CR2 (Canon RAW Version 2)
• NEF to TIFF
  NEF (Nikon Electronic Format)
• DNG to TIFF
  DNG (Digital Negative)
• PSD to TIFF
  PSD (Photoshop Document)
• RAW to TIFF
  RAW (RAW Image)
• CRW to TIFF
  CRW (Canon Raw Format)
• WMF to TIFF
  WMF (Windows Metafile)
• PCX to TIFF
  PCX (PiCture eXchange)
• DJVU to TIFF
  DjVu (DjVu Image)
• EPS to TIFF
  EPS (Encapsulated PostScript)
• DPX to TIFF
  DPX (Digital Picture Exchange)
• CBR to TIFF
  CBR (Comic Book RAR)
• CBZ to TIFF
  CBZ (Comic Book ZIP)
• WEBP to TIFF
  WEBP (Web Picture)
• HEIC to TIFF
  HEIC (High Efficiency Image Container)